Composite

Part:BBa_K2737009

Designed by: Xinyu Teng   Group: iGEM18_ECUST   (2018-10-06)


Pafe-GFP

Recombinant plasmid was transformed to E. coli DH5α and cultured at 37 °C for 12 h. Then positive bacteria is cultured in LB culture medium adding with 0.1% ampicillin. After bacteria grows to logarithmic phase, adding AHL until final concentration is10-4 ,10-5,10-6 ,10-7,10-8, 10-9 mol/L. Then light absorption is measured. Excitation/Emission wavelength is 485nm/530nm.

T--ECUST--b2.jpg

Figure 5 (a) Fluorescence intensity at different time induced by different concentrations of AHL. 

      (b)A three-dimensional map with log10 concentration of ahl,time and fluorescence intensity as the coordinate

When the AHL concentration is in the range of 10-7~10-8 mol/L, the fluorescence insensity was jump. When the AHL concentration is above 10-7 mol/L, the promoter will respond.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 35
    Illegal NheI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 734


[edit]
Categories
//function/reporter/fluorescence
Parameters
None